School of Biotechnology · Cell & tissue culture
Plant Tissue Culture & Micropropagation
Plant tissue culture is used for mass propagation, conservation and plant biotechnology. This course teaches the complete micropropagation workflow at the bench.
Course overview
Plant Tissue Culture & Micropropagation covers the tissue-culture laboratory, Murashige and Skoog media and plant growth regulators, and the surface sterilisation of explants, which decides whether cultures survive.
You initiate cultures, induce callus, multiply shoots, root them and plan hardening, and learn the record-keeping and contamination control used in commercial micropropagation units.
Learning outcomes
By the end of the course you will be able to:
- Prepare MS media with plant growth regulators
- Surface-sterilise explants and initiate aseptic cultures
- Induce callus and multiply shoots
- Root shoots and plan hardening
- Control contamination and keep culture records
Skills you will practise
Eligibility and prerequisites
Who can apply
- Students and graduates of B.Sc., M.Sc., B.Tech., M.Tech., B.Pharm., M.Pharm. or a PhD programme in biotechnology, microbiology, biochemistry, molecular biology, genetics, life sciences, pharmacy or allied health sciences
- Laboratory staff, research assistants and faculty who want structured bench training
- Participants must be 18 or older and follow the laboratory safety induction on day one
Prerequisites
- Undergraduate plant biology
Best suited to
- Students of botany, agriculture, horticulture and plant biotechnology
- Entrepreneurs exploring micropropagation
- Researchers in plant science
Module-wise syllabus
5 modules, each with a bench practical performed by every participant.
Topics
- Laboratory layout and laminar flow
- MS media and stock solutions
- Growth regulators
Topics
- Explant selection
- Surface-sterilisation protocols
- Initiation
Topics
- Auxin–cytokinin balance
- Callus induction
- Shoot regeneration
Topics
- Shoot multiplication
- Subculturing
- Rooting
Topics
- Acclimatisation
- Contamination control
- Production records
Practical sessions and projects
Practical assessment
Practical assessment
Maintain a micropropagation series through initiation, multiplication and rooting, with records and a contamination analysis.
Assessed on technique, accuracy, safety and the laboratory record. A pass is required for the certificate.
- The tissue-culture laboratory
Prepare MS stock solutions and media with growth regulators.
- Explants and sterilisation
Sterilise explants with two protocols and initiate cultures.
- Callus and organogenesis
Induce callus and set up regeneration media.
- Micropropagation
Subculture multiplying shoots and transfer shoots to rooting media.
- Hardening and quality
Plan hardening for rooted plantlets and audit contamination across your cultures.
Instruments, techniques and materials
Instruments and equipment
Techniques you will perform
- MS media preparation
- Explant sterilisation
- Callus induction
- Shoot multiplication
- In vitro rooting
- Hardening
Materials
- Common horticultural plant material
- MS medium components
- Growth regulators
Core reading
- Murashige T, Skoog F (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15:473–497.
- Bhojwani SS, Dantu PK. Plant Tissue Culture: An Introductory Text. Springer, 2013.
Duration, format and fees
- Next cohort
- Dates are sent with your admission offer
- Timetable
- Weekdays, 9:30 to 16:30, at our laboratory
- Payment
- Secure online payment link with your admission offer (UPI, cards, net banking). Instalments on request for fees above ₹15,000.
- Refunds
- See the refund and cancellation policy.
Instructor
The instructor for each cohort is named in your admission offer and on this page when the cohort is announced. Vedicgen instructors are selected for hands-on laboratory experience in the subject they teach, and every course is reviewed by a second subject expert.
Questions about who teaches a cohort? [email protected]
Certificate
Learners who complete the course and pass its practical assessment receive a Vedicgen certificate of completion for Plant Tissue Culture & Micropropagation, issued by Vivofold, stating the bench hours and techniques performed. Each certificate has a unique ID and QR code that anyone can check.
Apply for Plant Tissue Culture & Micropropagation
Apply
Send the form below. It takes about five minutes.
Review
An adviser confirms your fit and format within two working days.
Offer
You receive dates, timetable and a secure payment link.
Onboard
Safety briefing, lab address and joining instructions.
Frequently asked questions
Yes. Day one starts with a safety induction covering personal protective equipment, safe handling of reagents and biological material, spill response and waste segregation. All practicals use non-pathogenic material (Risk Group 1 organisms, established cell lines or synthetic templates) and are supervised throughout.
All reagents, kits, consumables, personal protective equipment, the lab manual and the certificate. Travel, accommodation and meals are not included. The laboratory address and joining instructions are sent with your admission letter.
Bench courses run in small groups so that every participant performs every practical personally, with an instructor-to-participant ratio that allows close supervision at the bench.
Yes. Learners who complete the course and pass its assessment receive a Vedicgen certificate of completion, issued by Vivofold. Every certificate carries a unique ID and a QR code that anyone, including an employer or admissions office, can check on the certificate verification page.
Apply with the form on this page. Live and in-person cohorts run through the year; the dates, timetable and joining details of the next cohort are sent with your admission offer and listed on this page once announced. Self-paced enrolment opens as soon as your application is accepted.
Yes. No course runs longer than four weeks, and the length is customisable: choose the standard schedule, an intensive one- to two-week schedule, weekends only, or a custom timetable. Pick your preference on the application form; colleges and teams can book their own dates.
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