Overview

What Sequence Studio does

Sequence Studio is a molecular-biology bench on screen. Find open reading frames, simulate restriction digests on a virtual gel, design primers and test them by in-silico PCR, plan Golden Gate and Gibson assemblies, and inspect Sanger traces against a reference.

It is built for the daily questions of wet-lab scientists and for teaching: every result is visual, every parameter is explained and nothing needs to be installed.

Technical specifications

Inputs: FASTA · Pasted text · Sanger .ab1 traces
Outputs: Annotated maps and gel images · Primer lists (CSV) · Assembled construct sequences (FASTA/GenBank-style) · Alignments
Engine: TypeScript kernels in a Web Worker
Platforms: Chrome, Edge, Firefox and Safari (current versions) · Windows, macOS, Linux and ChromeOS · No installation, no account, no upload
Limits: Browser-scale sequences; genome-wide searches belong on the future cloud tier
Licence: Free during the public preview; institutional licences on request

Features

Capabilities

01

Cloning simulator

Restriction, Golden Gate and Gibson assembly with primer design; invalid assemblies are refused with a reason.

02

Primers and in-silico PCR

Design primers with Tm, GC and dimer checks and confirm the products they would amplify.

03

Digest and virtual gel

Restriction maps and a gel image with ladders, so you know what to expect at the bench.

04

Sanger trace viewer

Open .ab1 files, trim by quality and compare with a reference.

05

Protein analysis

Hydropathy, transmembrane and disorder profiles, charge and composition.

06

Alignment and dot plots

Pairwise alignment with affine gaps and dot plots for repeats and rearrangements.

Use cases

Who uses it, and for what

Planning a cloning experiment
Checking primers before ordering
Verifying a construct from Sanger reads
Teaching molecular biology with real sequences

Documentation

Quick start

Open Sequence Studio
Use the button on this page or the Workbench menu. Nothing to install; works in current Chrome, Edge, Firefox and Safari.
Load data or the demo
Drop in your files (FASTA, Pasted text, Sanger .ab1 traces) or load the labelled synthetic demo to explore safely.
Run and inspect
Results appear as interactive figures and tables; each analysis records its parameters and versions.
Export and cite
Export figures and tables, add them to a report, and copy the auto-written methods paragraph.

Full documentation

Learn the methods with Vedicgen