School of Biotechnology · Bioprocess & analytical
Analytical Techniques: Spectroscopy, Chromatography & HPLC
Analytical techniques decide whether a sample is what it claims to be and how much of it there is. This course teaches the core spectroscopic and chromatographic methods used in pharmaceutical, food and biotech laboratories.
Course overview
Analytical Techniques covers UV–Vis spectroscopy, thin-layer and column chromatography, and the practical operation of an HPLC system: mobile phases, columns, detectors and integration.
You build calibration curves, run system-suitability tests and learn the ICH principles of method validation (specificity, linearity, accuracy, precision and robustness) used in regulated laboratories.
Learning outcomes
By the end of the course you will be able to:
- Perform quantitative UV–Vis analysis with calibration
- Separate compounds by TLC and column chromatography
- Operate an HPLC system and integrate chromatograms
- Run system-suitability tests
- Apply ICH method-validation parameters
Skills you will practise
Eligibility and prerequisites
Who can apply
- Students and graduates of B.Sc., M.Sc., B.Tech., M.Tech., B.Pharm., M.Pharm. or a PhD programme in biotechnology, microbiology, biochemistry, molecular biology, genetics, life sciences, pharmacy or allied health sciences
- Laboratory staff, research assistants and faculty who want structured bench training
- Participants must be 18 or older and follow the laboratory safety induction on day one
Prerequisites
- Undergraduate chemistry
Best suited to
- Students of pharmacy, biotechnology, chemistry and food science
- Graduates aiming at QC and analytical roles
- Researchers who need quantitative analysis
Module-wise syllabus
5 modules, each with a bench practical performed by every participant.
Topics
- UV–Vis principles
- Calibration and linearity
- Sources of error
Topics
- TLC and Rf values
- Column chromatography
- Fraction analysis
Topics
- Pumps, columns and detectors
- Mobile phases and gradients
- Peak integration
Topics
- External-standard calibration
- System-suitability parameters
- Troubleshooting
Topics
- ICH Q2 parameters
- Robustness
- Documentation
Practical sessions and projects
Practical assessment
Practical assessment
Quantify an analyte by HPLC with calibration and system suitability, and present a validation plan.
Assessed on technique, accuracy, safety and the laboratory record. A pass is required for the certificate.
- Spectroscopy
Quantify an analyte by UV–Vis with a calibration curve.
- Planar and column chromatography
Separate a mixture by TLC and column chromatography and analyse fractions.
- HPLC fundamentals
Run standards on an HPLC system and integrate the chromatograms.
- Quantification and suitability
Quantify an analyte in samples and evaluate system suitability.
- Method validation
Plan and partially execute a validation for the HPLC method.
Instruments, techniques and materials
Instruments and equipment
Techniques you will perform
- UV–Vis quantification
- TLC
- Column chromatography
- Reversed-phase HPLC
- System suitability
- Validation experiments
Materials
- Analytical standards
- HPLC-grade solvents
- Columns and consumables
Core reading
- Snyder LR, Kirkland JJ, Dolan JW. Introduction to Modern Liquid Chromatography, 3rd edition. Wiley, 2010.
- ICH Q2(R2): Validation of Analytical Procedures (current guideline).
Duration, format and fees
- Next cohort
- Dates are sent with your admission offer
- Timetable
- Weekdays, 9:30 to 16:30, at our laboratory
- Payment
- Secure online payment link with your admission offer (UPI, cards, net banking). Instalments on request for fees above ₹15,000.
- Refunds
- See the refund and cancellation policy.
Instructor
The instructor for each cohort is named in your admission offer and on this page when the cohort is announced. Vedicgen instructors are selected for hands-on laboratory experience in the subject they teach, and every course is reviewed by a second subject expert.
Questions about who teaches a cohort? [email protected]
Certificate
Learners who complete the course and pass its practical assessment receive a Vedicgen certificate of completion for Analytical Techniques: Spectroscopy, Chromatography & HPLC, issued by Vivofold, stating the bench hours and techniques performed. Each certificate has a unique ID and QR code that anyone can check.
Apply for Analytical Techniques: Spectroscopy, Chromatography & HPLC
Apply
Send the form below. It takes about five minutes.
Review
An adviser confirms your fit and format within two working days.
Offer
You receive dates, timetable and a secure payment link.
Onboard
Safety briefing, lab address and joining instructions.
Frequently asked questions
Yes. Day one starts with a safety induction covering personal protective equipment, safe handling of reagents and biological material, spill response and waste segregation. All practicals use non-pathogenic material (Risk Group 1 organisms, established cell lines or synthetic templates) and are supervised throughout.
All reagents, kits, consumables, personal protective equipment, the lab manual and the certificate. Travel, accommodation and meals are not included. The laboratory address and joining instructions are sent with your admission letter.
Bench courses run in small groups so that every participant performs every practical personally, with an instructor-to-participant ratio that allows close supervision at the bench.
Yes. Learners who complete the course and pass its assessment receive a Vedicgen certificate of completion, issued by Vivofold. Every certificate carries a unique ID and a QR code that anyone, including an employer or admissions office, can check on the certificate verification page.
Apply with the form on this page. Live and in-person cohorts run through the year; the dates, timetable and joining details of the next cohort are sent with your admission offer and listed on this page once announced. Self-paced enrolment opens as soon as your application is accepted.
Yes. No course runs longer than four weeks, and the length is customisable: choose the standard schedule, an intensive one- to two-week schedule, weekends only, or a custom timetable. Pick your preference on the application form; colleges and teams can book their own dates.
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